immunoreagents Conjugates說明書

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Conjugate
Notes
HRP is an enzyme found in the roots of horseradish. It is used
Horseradish Peroxidase
in many immunoassays including ELISA's. HRP reacts with chro-
(HRP)
mogenic substrates TMB, DAB, and ABTS along with luminol in
enhanced chemiluminescence (ECL) assays.
Alkaline Phosphatase
ALP is an enzyme derived from calf intestine that is commonly
(ALP)
used in ELISA and Western Blot assays. Common substrates
include NBT, BCIP, pNPP, and MUP.
Fluorescein lsothocyanate
FITC is a green colored fluorescein derivative used in wide-
(FITC)
ranging applications including flow cytometry and IHC. FITC has
Ex/Em spectrum peak wavelengths 488 nm / 519 nm.
DyLighte fluorescent probes are complete family of high inten-
DyLight@
sity, photostable fluorescent tags. They are used in flow cytom-
etry, ELISA, and fluorescence microscopy.
(See our available Fluorophores below)*
DyLight@ 350 (Ex = 353 nm;
DyLight8 633 (Ex = 638 nm;
Em = 432 nm)
DyLight@ 405 (Ex = 400 nm;
Em = 658 nm)
DyLight@ Fluorophores
Em= 420 nm)
DyLight@ 650 (Ex = 652 nm;
DyLight@ 488 (Ex = 493 nm;
Em= 672 nm)
Available*
Em= 518 nm)
DyLight8 680 (Ex = 682 nm;
Em= 715 nm)
DyLight@ 550 (Ex = 562 nm;
DyLight@ 800 (Ex = 770 nm;
Em = 576 nm)
DyLight@ 594 (Ex = 593 nm,
Em= 794 nm)
Em= 618 nm)
Biotin is a water soluble B vitamin that can be attached to an-
Biotin
tibodies with linkers. Biotinylated antibodies in combination
with Streptavidin, Avidin, or Neutravidin.
TRITC is a bright orange fluorescent dye ideally matched with
Tetramethylrhodamine the 532 laser line. It is used in cellular imaging such as fluo-
(TRITC)
rescence microscopy. TRITC has Ex/Em spectrum peak wave-
lengths 550 nm / 570 nm.